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19:20
YouTube
Noor Biotech Institute (NBI)
How to Design sgRNA for CRISPR-Cas9 Gene Editing: A Beginner's Walkthrough
Designing a good sgRNA is one of the most important steps in any CRISPR-Cas9 experiment, and it can feel intimidating the first time you try it. In this short lecture, I walk through the basics of sgRNA design so you can see the workflow from start to finish. I show you how to paste a gene sequence, select a target region, and read the output ...
3.7K views
Jun 19, 2022
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